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HPLC-Principle, Instrumentation, Parameters And Applications

19+ Sessions

1 hr 35 min 49 sec

2 Students enrolled

Pharmacy
Img
Lakshmi Aswini Ganipisetty

Instructor

Overview

The Contents Of The Online Course Are Listed Below:

1. Introduction To Author, Contents Of HPLC [High Performance Liquid Chromatography].

2. About HPLC.

3. Types Of HPLC Techniques And Principle Of HPLC.

4. Instrumental Requirements – HPLC Pumps, Different Types Of HPLC Pumps.

5. Mixing Unit, Gradient Controller, Solvent Degassing.

6. Injectors And Their Types - Septum Injectors, Stop Flow Injectors, Rheodyne Injectors.

7. Columns And Their Types- Guard Columns And Analytical Columns.

8. Types Of Columns - Normal Phase Columns, Reverse Phase Columns, Ion Exchange Columns And Size Exclusion Columns

9. Detectors And Their Types.

10. Types Of Detectors - UV/Visible Detectors, RI Detectors, Fluorescence Detectors, Electrochemical Detectors, Conductivity Detectors

11. Types Of Detectors - Evaporating Light Scattering Detectors, Mass Detector, Ir Detector And Optical Rotation Detector

12. Recorders And Integrators.

13. Parameters Used In HPLC, Retention Time, Retention Volume

14. Resolution And Separation Factor

15. Theoretical Plates

16. Asymmetric Factor

17. Applications Of HPLC

18. Quantitative Analysis

19. Multi-Component Analysis

Course Content
19 Sessions 1 hr 35 min 49 sec

  • ●

    Introduction, Contents Of HPLC

    Preview

    02:46

  • ●

    About HPLC

  • ●

    Types Of HPLC Techniques And Principle Of HPLC

  • ●

    Instrumental Requirements

  • ●

    Mixing Unit, Gradient Controller, Solvent Degassing

  • ●

    Injectors And Their Types

  • ●

    Columns And Their Types

  • ●

    Types Of Columns

  • ●

    Detectors And Their Types

  • ●

    Types Of Detectors (Con't)

  • ●

    Types Of Detectors (Con't)

  • ●

    Recorders And Integrators

  • ●

    Parameters Used In HPLC, Retention Time And Retention Volume

  • ●

    Resolution And Separation Factor

  • ●

    Theoritical Plates

  • ●

    Asymmetric Factor

  • ●

    Applications Of HPLC

  • ●

    Quantitative Analysis

  • ●

    Multi Component Analysis

About the instructor
Img
Lakshmi Aswini Ganipisetty

Online Instructor

2+ Courses

6+ Students enrolled

I have completed M.Pharmacy and pursuing Ph.D, completed Diploma in Nutrition and having 15 years of teaching experience in reputed Pharmacy Colleges. I have published 30 research papers in various International Journals and review Articles.

I have attended seminars and workshops in various reputed colleges and attended Indian Pharmacy Council (IPC).

HPLC-Principle, Instrumentation, Parameters and Applications

₹ 499.00

₹ 999.00

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Includes
  • ● Introduction to Author, Contents of HPLC [High performance liquid chromatography]
  • ● About HPLC
  • ● Types of HPLC Techniques and Principle of HPLC
  • ● Instrumental requirements – HPLC Pumps, different types of HPLC pumps
  • ● Mixing Unit, gradient controller, solvent degassing
  • ● Injectors and their types - Septum injectors, Stop flow injectors, Rheodyne injectors
  • ● Columns and their types- Guard Columns and Analytical Columns
  • ● Types of Columns - Normal phase columns, Reverse phase columns, Ion exchange columns and Size exclusion columns
  • ● Detectors and their types
  • ● Types of Detectors - UV/Visible detectors, RI detectors, fluorescence detectors, electrochemical detectors, conductivity detectors
  • ● Types of Detectors - evaporating light scattering detectors, mass detector, Ir detector and optical rotation detector
  • ● Recorders and Integrators
  • ● Parameters used in HPLC, Retention Time, Retention Volume
  • ● Resolution and Separation factor
  • ● Theoretical Plates
  • ● Asymmetric Factor
  • ● Applications of HPLC
  • ● Quantitative Analysis
  • ● Multi-Component Analysis
Course Features

  • Enrolled: 2 Students

  • Topics: 1

  • Video: 1 hr 35 min 49 sec